TY - JOUR ID - 29896 TI - Ameliorative Effects of He:Ne Laser Irradiation on DNA Damage of Blood of Children with Acyanotic Heart Disease JO - The International Conference on Mathematics and Engineering Physics JA - ICMEP LA - en SN - 2636-431X AU - Abdel Salam, Ekram AU - El Shinawy, Rabha AU - Korraa, Soheir AU - Abdel Meguid, Iman E. AU - El Sharnouby, Gihan AD - Y1 - 2008 PY - 2008 VL - 4 IS - International Conference on Mathematics and Engineering Physics (ICMEP-4) SP - 1 EP - 13 DO - 10.21608/icmep.2008.29896 N2 - AbstractLow-energy laser irradiation (LELI) has been found to attenuate various biologicalprocesses in tissue culture and experimental animal models. The aim of the present study wasto investigate the role of LELI in ameliorating markers of oxidative stress (in terms of bloodantioxidant enzymes levels, NO generation, apoptotic genes together with DNAfragmentation and apoptosis percentage in circulating lymphocytes) in cyanotic and acyanoticcongenital heart diseases with reference to normal healthy children. Accordingly, wholeblood from 16 cyanotic, 14 acyanotic and 15 controls was exposed to 8 mW He-Ne laser atenergy densities of 3 J/ cm2 and examined before, after and without laser exposure 24 hourspost irradiation for levels blood antioxidant enzymes, NO generation, Bax RNA and Bcl2protein together with DNA fragmentation and apoptosis percentage in circulatinglymphocytes. Results indicated that there were significant increases in mean ± SD of plasmanitrite, MDA, DNA fragmentation and apoptosis in circulating lymphocytes of both acyanoticand cyanotic cases compared to controls before laser irradiation. Laser ameliorated oxidativestress in acyanotic heart disease blood, but increased apoptosis and DNA fragmentation incyanotic cases 24 hours post laser irradiation. In control cases laser had a biostimulatoryeffect exhibited by decreased lipid peroxidation, apoptosis and DNA fragmentationcompared to its level without laser irradiatioin. Also, there was significant increase in plasma  nitrite in controls, cyanotic and acyanotic cases 24 hours post irradiation with laser compared to their level without laser irradiation. However, cyanotic cases showed the highestsignificant value and were significantly higher when compared to both cyanotic and acyanoticcases. It is concluded that He:Ne laser have an ameliorative effect on damaging effectsinduced due cardiac catheterization procedure in case of acyanotic congenital heart diseases,but it worsen the effects in cyanotic congenital heart diseases. Increased nitric oxideproduction seems to be the reason for the increased oxidative stress in cyanotic heart diseases,which are worsened when exposed to laser irradiation. UR - https://icmep.journals.ekb.eg/article_29896.html L1 - https://icmep.journals.ekb.eg/article_29896_5949752624b65bfd1cce4ad2c9182afc.pdf ER -