Nanoparticles Photosensitization in Sebaceous Gland

Aِbstract
Aim of Study: Current topical and most oral therapies for sebaceous glands have limited
efficacy because drugs active constituents do not reach the sebaceous gland efficiently. Most
recently it has been shown laser-induced flow in nanoscale cavities could represent a
successful mechanism low level laser therapy.
Aim of the work: The aim of the present study is to investigate, whether a coordinate
combination betweeen laser therapy and methylene blue delivered by liposomes or gel can be
effective in destructing sebaceous glands
Materials and Methods: Gel or liposome preparations of Methylene blue were topically
applied on mice skin. After being absorbed into skin methylene blue was exposed to different
fluencies of He:Ne laser and skin biopsies were obtained and examined histiologically.
Results: Liposome delivery system showed significantly increased diffusion of methylene
blue into the pilosebaceous unit and deeper skin layers compared to gel delivered methylene
blue. It was evident that destruction of the pilosebaceous unit depends on laser parameters and
the on lag time between laser application and biopsy sampling.
Conclusion: Temporal coordination between low level laser treatment and liposome
delivered methylene blue drug system maximizes drug effects and minimizes possible adverse
effects. Irradiation should start when the drug concentration has reached its maximum in the
desired field of action